Study on the Protective Immunity Induced by Pseudotyped Baculovirus Expressing the E Protein of Tembusu Virus in Ducklings

The Duck Tembusu virus (DTMUV), a pathogenic flavivirus, has been causing significant economic losses in the Chinese poultry industry since 2010. This can severely decrease egg production and inhibit growth of laying ducks ducklings. While many vaccines have developed to prevent DTMUV infection, fresh outbreaks continue occur, as few effective are available. E glycoprotein is primary target for inducing protective immunity natural host. Therefore, we conducted an investigation successfully recombinant baculovirus containing gene. Ducklings were then vaccinated with purified protein derived from this potential vaccine candidate. Our findings demonstrated that was highly expressed Sf9 cells. vaccination ducklings Bac-E resulted induction strong humoral cellular immune responses. Most significantly, observed provided 100% against lethal challenges YY5 strain.